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OBJECTIVE@#To explore the protective effect of SBi4211 (heptamidine), an inhibitor of S100B, against central nervous system injury induced by HIV-1 envelope protein gp120.@*METHODS@#In an @*RESULTS@#In the cell co-culture system, SBi4211 treatment significantly inhibited gp120-induced expression of S100B, RAGE and GFAP in U251 cells (@*CONCLUSIONS@#SBi4211 can protect neurons from gp120-induced neurotoxicity possibly by inhibiting the S100B/ RAGE-mediated signaling pathway.
Subject(s)
Animals , Mice , Astrocytes , Blotting, Western , Central Nervous System , HIV Envelope Protein gp120 , Neurons , S100 Calcium Binding Protein beta Subunit , Signal TransductionABSTRACT
Steroid hormones are fat soluble molecules produced by cholesterol through a series of enzymatic catalysis.These low-level steroid hormones in body fluids generally play an important role in the regulation of metabolism and sexual function.Therefore,accurate quantification of these hormones is crucial for both diagnosis and treatment of endocrine disorder disease.At present, automated immunoassays are commonly used for the measurement of steroid hormone.However,only one analyte can be detected in one assay.More importantly, a lot of steroid hormones still cannot be detected by immunoassays due to the limitation of specific antibody.Liquid chromatography tandem mass spectrometry(LC-MS/MS)has high sensitivity and high specificity,and can simultaneously detect a variety of steroid hormones in one injection. LC-MS/MS has become a hot research topic in clinical research.
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Objective:To assess the possibility of suicide in setting condition among urban and rural residents samples in northern China.Methods:By randomly sampling,1007 persons aged 18 years or older were selected from 10 urban neighborhoods in Beijing and from 10 rural village in Hebei province.The Survey Schedule of Atti-tudes in China in which 24 negative life events were listed,and the possibility of committing suicide was asked to the subjects when they encountered the supposed events.Results:The rates of thinking about committing suicide when encountered the supposed life events were 65.2% of incurable illness,62.0% of burden on other and no future hope,61.2% of drug dependence,57.3% of large debt due to gambling,54.2% of being raped, 51.6% of severe depression,50.7% of being elderly and no family to provide support.Only 14.6% of the ur-ban and rural residents would not consider suicide when encountered any type of supposed problems.Conclusion:There is higher possibility of considering suicide in urban and rural residents when encountered negative life events, so it is important to give support to the residents with one or more life events.
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Objective:Describe the prevalence of depressive disorder and the care-seeking of depressed individuals among inpatients in general hospitals in Beijing.Methods:2 925 inpatients ≥15 years of age from 40 general hospitals (selected by stratified random sampling) were first screened by psychiatric nurses and then psychiatrists used DSM-IV criteria to generate a 'gold standard' diagnosis in subjects screened positive and 10% of those screened negative. Results:Adjusted point prevalence, one-year prevalence, and lifetime prevalence of any type of depressive disorder were 7.04%, 7.47% and 9.96%, respectively; corresponding prevalences of Major Depressive Disorder were 3.83%, 4.33%, and 6.11%, respectively. Of the 123 depressed patients only 12 (9.8%) had ever received treatment. The prevalence was significantly higher in females than in males(5.18%vs 3.59%,RR=1.44 95%CI:1.01~2.05), in patients from Level-2 hospitals than in those from Level-3 hospitals(6.28%vs 3.31%,RR=1.90 95%CI:1.31~2.75), and in patients on internal medicine wards than in those from surgery (6.08%vs3.60%,RR=1.69 95%CI:1.15~2.49)or other wards(6.08%vs 3.01%,RR=2.02 95%CI:1.21~3.37).Conclusions:The prevalence of depressive disorder among inpatients in Beijing's general hospitals is high but very few are diagnosed or treated. Different programs to increase the recognition and treatment of depression in general hospitals need to be developed and regularly monitored.